Journal: Malaria Journal

Article Title: RDL mutations in Guangxi Anopheles sinensis populations along the China–Vietnam border: distribution frequency and evolutionary origin of A296S resistance allele

doi: 10.1186/s12936-020-3098-y

Figure Lengend Snippet: Diagnostic PCR-RFLP tests to detect the T345S mutation. The 167 bp amplicon is undigested by HpyCH4 III for homozygous TCG (mutant 345SS), and cut into two fragments of 83 bp and 84 bp for homozygous ACG (wild 345TT) that co-migrate in the agarose gel and cannot be distinguished. A combined pattern (two bands 167 bp + 83/84 bp) is displayed for heterozygotes

Article Snippet: The reaction procedure was set as 95 °C for 5 min; 38 cycles of 95 °C for 30 s, 56 °C for 30 s, 72 °C for 10 s; and 72 °C for 10 min. PCR products were digested with the restricted DNA digestion enzyme HpyCH4 III (New England Biolabs) (Fig. a) for 2 h in a total volume of 20 μl according to the manufacturer’s instruction.

Techniques: Diagnostic Assay, Mutagenesis, Amplification, Agarose Gel Electrophoresis

Journal: Indian Journal of Microbiology

Article Title: Development of an Accurate and Sensitive Diagnostic System Based on Conventional PCR for Detection of African Swine Fever Virus in Food Waste

doi: 10.1007/s12088-022-01007-y

Figure Lengend Snippet: Specific, non-specific, and sensitivity tests for the PCR primer sets developed for the detection of African swine fever virus. 100 bp DNA ladder marker (Enzynomics, Korea). Panel A: Specific reactions; V, selected primer sets. Panel B: Non-specific reactions of the reference viruses. ASFV, African swine fever virus ; PCV2, porcine circovirus 2; PPV, porcine parvovirus ; PrV, pseudorabies virus Panel C: Sensitivity test –4 to –9, dilution value for the template from ASFV plasmid concentration of 1 ng/μL; N, negative control

Article Snippet: Fig. 4 African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Techniques: Virus, Marker, Plasmid Preparation, Concentration Assay, Negative Control

Journal: Indian Journal of Microbiology

Article Title: Development of an Accurate and Sensitive Diagnostic System Based on Conventional PCR for Detection of African Swine Fever Virus in Food Waste

doi: 10.1007/s12088-022-01007-y

Figure Lengend Snippet: African swine fever virus (ASFV) monitoring in twenty food waste samples (panel A) and sensitivity tests using ASFV artificial infections (panel B) M, 100 bp DNA ladder marker (Enzynomics); P, positive control; N, negative control; 1–20, number of food waste samples; –1 to –8; concentrations of ASFV plasmid in the artificial infection samples

Article Snippet: Fig. 4 African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Techniques: Virus, Marker, Positive Control, Negative Control, Plasmid Preparation, Infection

Journal: Indian Journal of Microbiology

Article Title: Development of an Accurate and Sensitive Diagnostic System Based on Conventional PCR for Detection of African Swine Fever Virus in Food Waste

doi: 10.1007/s12088-022-01007-y

Figure Lengend Snippet: Comparison of specific and non-specific reactions for the twenty food waste samples and the artificial infections based on the sensitivity tests for the five reference PCR primer sets used for the detection of African swine fever virus (ASFV). M, 100 bp DNA ladder marker (Enzynomics); P, positive control; N, negative control; PCV2, porcine circovirus 2; PPV, porcine parvovirus ; PrV, pseudorabies virus ; 1–20, number of food waste samples; –1 to –8; concentration of ASFV nucleic acid artificial infection samples [ , , – ]

Article Snippet: Fig. 4 African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Techniques: Comparison, Virus, Marker, Positive Control, Negative Control, Concentration Assay, Infection

Journal: Indian Journal of Microbiology

Article Title: Development of an Accurate and Sensitive Diagnostic System Based on Conventional PCR for Detection of African Swine Fever Virus in Food Waste

doi: 10.1007/s12088-022-01007-y

Figure Lengend Snippet: African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Article Snippet: Fig. 4 African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Techniques: Virus, Modification, Marker

Journal: Indian Journal of Microbiology

Article Title: Development of an Accurate and Sensitive Diagnostic System Based on Conventional PCR for Detection of African Swine Fever Virus in Food Waste

doi: 10.1007/s12088-022-01007-y

Figure Lengend Snippet: Validation result of developed ASFV PCR diagnostic system in this study based on reproducibility M, 100 bp DNA ladder marker (Enzynomics); P, positive control; N, negative control; -1 ~ -8, dilution value for the template from ASFV plasmid concentration of 1 ng/μL; 1–10, sample number of analytical quality control (AQC) test

Article Snippet: Fig. 4 African swine fever virus (ASFV) detection using the developed PCR primer set and modified positive controls and the restriction enzyme Eco RV digestion 100 bp DNA ladder marker (Enzynomics)

Techniques: Biomarker Discovery, Diagnostic Assay, Marker, Positive Control, Negative Control, Plasmid Preparation, Concentration Assay, Control